Malaysian Journal of Analytical Sciences Vol 25 No 2 (2021): 193 - 202

 

 

 

 

BIODEGRADATION OF OIL AND GREASE FROM AGRO-FOOD INDUSTRY BY IMMOBILISED Serratia marcescens SA30

 

(Biodegradasi Minyak dan Gris dari Industri Agro-Makanan oleh Serratia marcescens SA30 yang Dipegunkan)

 

Shakila Abdullah¹_*, Farhah Husna Mohd Nor¹, Mohd Hairul Khamidun²

 

¹Department of Physics and Chemistry, Faculty of Applied Sciences and Technology,

Universiti Tun Hussein Onn Malaysia, Educational Hub Pagoh, 84600 Muar, Johor, Malaysia

²Department of Civil Engineering, Faculty of Civil Engineering and Built Environment,

Universiti Tun Hussein Onn Malaysia, 86400 Batu Pahat, Johor, Malaysia

 

*Corresponding author:  shakilaa@uthm.edu.my

 

 

Received: 20 July 2020; Accepted: 10 March 2021; Published:  25 April 2021

 

 

Abstract

The agro-food industrial wastewater (AFIW) contains high concentrations of oils and grease (O&G), which are significant threats to aquatic environments. In the context of the removal of contaminants from wastewater, the capability of Serratia marcescens SA30 immobilized in a packed-bed column reactor (PBCR) of O&G removal from AFIW needs to be verified. This study analyses the Serratia marcescens SA30 immobilized on oil palm frond (OPF) in PBCR in order to elucidate its removal ability of O&G from AFIW. The physicochemical parameters of the AFIW samples collected from the agro-food industry were analyzed according to Standard Methods for Examination of Water and Wastewater. The PBCR treatment system was set up using immobilized Serratia marcescens SA30 onto OPF for the removal of O&G from AFIW. The AFIW samples were collected at the inlet and outlet of the PBCR, and the respective concentrations of O&G were determined. These values assert that the parameters does not comply the production limit set in Environmental Environment Quality B (Industrial Effluent Regulations, 2009). The performance of the PBCR realized 100% efficiency, with the population ranging from 10⁸ – 10⁷ with the immobilized Serratia marcescens SA30 acting as a biosurfactant-producing bacteria, which was achieved by experiments ran at a volumetric flow rate of 3 mL/min during treatment using concentrations of O&G at 100% v/v after 144 hours operation in the PBCR. The data obtained would provide a green and sustainable pathway for the removal of O&G from water.

 

Keywords:  Agro-food industrial wastewater, packed-bed column reactor, Serratia marcescens SA30, oil and grease, immobilized

 

Abstrak

Sisa air perindustrian agro-makanan (AFIW) mengandungi kepekatan minyak dan gris (O&G) yang boleh memberikan ancaman kepada persekitaran akuatik. Dalam konteks penyingkiran bahan bukan organik dan organik dari air sisa, keupayaan Serratia marcescens SA30 yang dipegunkan dalam reaktor turus terpadat tunggal (PBCR) perlu disahkan. Kajian ini menganalisis Serratia marcescens SA30 yang dipegunkan di pelepah kelapa sawit (OPF) dalam PBCR untuk menjelaskan kemampuan penyingkiran O&G dari AFIW. Parameter fizikokimia sampel AFIW yang diambil dari industri agro-makanan dianalisis berdasarkan Kaedah Piawai untuk Pemeriksaan Air dan Air Sisa. Sistem rawatan PBCR dibentuk menggunakan Serratia marcescens SA30 yang dipegunkan ke OPF untuk penyingkiran O&G dari AFIW. Sampel AFIW dikumpulkan di saluran masuk dan keluar PBCR, dan kepekatan O&G masing-masing ditentukan. Nilai-nilai tersebut menegaskan bahawa parameter tersebut tidak mencapai limit pengeluaran yang telah ditetapkan dalam Kualiti Persekitaran Lingkungan B (Peraturan Efluen Industri, 2009). Prestasi PBCR mencapai kecekapan 100% dengan populasi antara 10⁸-10⁷ dengan Serratia marcescens SA30 yang dipegunkan bertindak sebagai bakteria penghasil biosurfaktan, yang dicapai dengan eksperimen yang dijalankan pada kadar aliran volumetric 3 mL/min semasa rawatan menggunakan kepekatan O&G pada 100% v/v setelah 144 jam beroperasi dalam PBCR. Kajian ini akan memberikan laluan hijau dan lestari untuk menyingkirkan O&G dari air.

 

Kata kunci: Sisa air perindustrian agro-makanan, reaktor turus terpadat tunggal, Serratia marcescens SA30, minyak dan gris, dipegunkan

 

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