Malaysian Journal of Analytical Aciences Vol 18 No 2
(2014): 251 – 259
SYNTHESIS, STRUCTURAL AND ANTIBACTERIAL STUDIES OF NEW DITHIOCARBAMATE
COMPLEXES OF Sb(III) AND Bi(III)
(Sintesis, struktur dan kajian
antibakteria kompleks baru ditiokarbamat Sb(III) dan Bi(III))
Nur Amirah
Jamaluddin1, Ibrahim Baba1*, Nazlina Ibrahim2
1School
of Chemical Sciences and Food Technology,
2School
of Biosciences and Biotechnology,
Faculty of
Science and Technology,
Universiti
Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
*Corresponding author: aibi2005@gmail.com
Abstract
Six new dithiocarbamate
complexes from three different amines, e.g. N-ethylethanol-, N-butylethyl- and
N-benzylmethylamine were successfully
prepared using in situ method. All complexes were characterized by
elemental analysis, IR, UV-Vis, 13C and 1H NMR. Elemental
analysis data (C, H, N and S) showed an agreement with the general formula of
MCl(S2CNR’R’’)2, (M = Sb(III), Bi(III); R’ = ethyl, butyl
and benzyl; R” = ethanol, ethyl and methyl). The complexes had been
characterized by infrared spectroscopy that showed a thioureide bands, n(C
N) in the region of
1427 - 1490 cm-1 followed by n(C
S) bands that can
be seen in the region of 935 - 1060 cm-1 and ν(M-S) bands
existed in the region of 350 - 392 cm-1. Maximum wavelength
absorption for ultraviolet-visible spectroscopy for N
C
S group was at 254
nm which indicates π→π* transition. Data from 13C
NMR showed a signal in the region of 198.39 – 199.44 ppm that corresponded to
the NCS2 group. The crystal structure of bis(N,N’-butylethyldithiocarbamato)chlorido
antimony(III), SbCl[S2CN(C4H9)(C2H5)]2
demonstrated a five-coordination geometry, triclinic system, space group P1
with a = 10.0141(8) Å, b = 10.1394(7) Å, c = 11.8665(9) Å,
α = 67.960(2)°, β = 87.616(2)° and γ = 80.172(2)°. Minimum
inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)
tests were done using five dithiocarbamate complexes which are BiCl[S2CN(C2H5)(C2H4OH)]2,
SbCl[S2CN(C2H5)(C2H4OH)]2,
BiCl[S2CN(C4H9)(C2H5)]2,
SbCl[S2CN(C4H9)(C2H5)]2,
BiCl[S2CN(C7H7)(CH3)]2
towards S. aureus, S. epidermidis, E. aerogenes and E. coli.
It was found that all four complexes were active against S. aureus except
SbCl[S2CN(C2H5)(C2H4OH)]2
while BiCl[S2CN(C7H7)(CH3)]2
and BiCl[S2CN(CH3)(C6H11)]2
complexes were active against S. epidermidis with MIC value of 5.0 mg/mL
and 2.5 mg/mL respectively and MBC value of 5.0 mg/mL for both compounds. Only
BiCl[S2CN(C2H5)(C2H4OH)]2
was active against E. aerogenes with MIC, 1.25 mg/mL and MBC, 10 mg/mL.
All complexes were less active as antibacterial agent against E. coli strain.
Keywords:
Dithiocarbamate, antimony(III), bismuth(III), antibacterial
Abstrak
Enam
sebatian ditiokarbamat baru daripada tiga jenis amina yang berbeza, iaitu
N-etilaminoetanol, N-butiletil, N-benzilmetil telah berjaya disediakan dengan
menggunakan kaedah in situ. Kesemua sebatian telah dicirikan dengan
analisis unsur, IR, UV-Vis, 13C dan 1H NMR. Data analisis
unsur (C, H, N dan S) bagi kesemua sebatian menepati formula umum MCl(S2CNR’R’’)2 (M =
Sb(III), Bi(III); R’ = etil, butil dan benzil; R” = etanol, etil dan metil).
Kompleks dicirikan menggunakan kaedah spektroskopi inframerah menunjukkan jalur
tioreida, n(C
N)
berada dalam julat 1427 - 1490 cm-1 diikuti jalur n(C
S) yang
diperhatikan dalam julat 935 - 1060 cm-1 dan jalur logam sulfur,
ν(M-S) berada dalam julat 350 - 392 cm-1. Serapan jarak
gelombang maksimum untuk spektroskopi ultralembayung-nampak untuk kumpulan N
C
S ialah pada 254
nm yang menunjukkan peralihan π→π*. Data daripada 13C
NMR menunjukkan isyarat dalam julat 198.39 – 199.44 ppm merujuk kepada kumpulan
NCS2. Struktur hablur bis(N,N’-butiletilditiokarbamato)klorido
stibium(III), SbCl[S2CN(C4H9)(C2H5)]2
menunjukkan geometri lima koordinatan, sistem triklinik, kumpulan ruang P1 dengan a = 10.0141(8) Å,
b = 10.1394(7) Å, c = 11.8665(9) Å, α = 67.960(2)°, β =
87.616(2)° and γ = 80.172(2)°. Ujian kepekatan minimum perencatan (MIC)
dan kepekatan minimum bakterisidal (MBC) telah dijalankan menggunakan lima
sebatian ditiokarbamat, iaitu BiCl[S2CN(C2H5)(C2H4OH)]2,
SbCl[S2CN(C2H5)(C2H4OH)]2,
BiCl[S2CN(C4H9)(C2H5)]2,
SbCl[S2CN(C4H9)(C2H5)]2,
BiCl[S2CN(C7H7)(CH3)]2
terhadap S. aureus, S. epidermidis, E. aerogenes and E. coli.
Kesemua empat sebatian adalah aktif terhadap S. aureus kecuali SbCl[S2CN(C2H5)(C2H4OH)]2
manakala sebatian BiCl[S2CN(C7H7)(CH3)]2
dan BiCl[S2CN(CH3)(C6H11)]2
aktif terhadap S. epidermidis dengan nilai MIC masing-masing ialah 5.0
mg/mL dan 2.5 mg/mL manakala nilai MBC 5.0 mg/mL untuk kedua-dua sebatian.
Hanya sebatian BiCl[S2CN(C2H5)(C2H4OH)]2
yang aktif terhadap E. aerogenes dengan nilai MIC, 1.25 mg/mL dan nilai
MBC, 10 mg/mL. Kesemua sebatian kurang aktif sebagai agen antibakteria terhadap
strain E. coli.
Kata kunci:
Ditiokarbamat, stibium(III), bismuth(III), antibakteria
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