Malaysian Journal of Analytical Sciences, Vol 27 No 3 (2023): 463 - 470

 

THE DEVELOPMENT AND QUANTITATIVE PERFORMANCE TEST OF LOW-COST VISIBLE SPECTROPHOTOMETER AND ITS COMPARISON WITH COMMERCIAL SPECTROPHOTOMETER

 

(Pembangunan dan Ujian Prestasi Kuantitatif dari Spektrofotometer Nampak Kos Rendah dan Perbandingannya dengan Spektrofotometer Komersil)

 

Rudi Heryanto1,2, Dyah Iswantini1,2*, Eti Rohaeti1,2, Mohamad Rafi1,2, Mulyati1, and Wulan Suci Pamungkas1

 

1 Department of Chemistry,

Faculty of Mathematics and Sciences

2 Tropical Biopharmaca Research Center,

Institute for Research and Community Service

IPB University, Bogor 16680, Indonesia

 

*Corresponding author: dyahis@apps.ipb.ac.id

 

 

Received: 13 December 2022; Accepted: 1 April 2023; Published:  23 June 2023

 

 

Abstract

A simple, portable, visible spectrophotometer was constructed and used for analytical measurements. The spectrophotometer was built using consumer electronics components such as an LED as the light source and a web camera as the detector. Using parameters including linearity, limit of detection, quantification, and precision to analyze the methyl red and dichromate standards, the analytical performance of the webcam spectrophotometer was evaluated. Results showed that, for methyl red, the webcam spectrophotometer gave a linearity range of 2–10 ppm with a limit of detection and limit of quantification of 0.69 and 2.11 ppm, respectively, and precision as a relative standard deviation of approximately 5%. The dichromate standard gave a linearity range of 0.7–1.5 ppm with a limit of detection and limit of quantification of 0.12 and 0.41 ppm, respectively, and precision as a relative standard deviation under 5%. These analytical performances are comparable but not better than those of the two commercial spectrophotometers used as comparators. The application of a webcam spectrophotometer to quantify the rhodamine B content in a traditional food sample, pacar cina jelly, also gave an equivalent analytical performance, and the rhodamine B analyte quantification capability was not significantly different from that of commercial spectrophotometers.

 

Keywords:  visible spectrophotometry, webcam spectrophotometer, analytical performance, quantitative analysis

 

Abstrak

Spektrofotometer nampak dan mudah alih telah dibina dan digunakan untuk pengukuran analitik. Spektrofotometer dibina menggunakan komponen elektronik pengguna seperti LED sebagai sumber cahaya dan kamera web sebagai pengesan. Menggunakan parameter termasuk kelinearan, had pengesanan, kuantifikasi dan ketepatan untuk menganalisis piawaian metil merah dan dikromat, prestasi analisis spektrofotometer kamera web telah dinilai. Keputusan menunjukkan bahawa, untuk metil merah, spektrofotometer kamera web memberikan julat lineariti 2-10 ppm dengan had pengesanan dan had kuantifikasi masing-masing 0.69 dan 2.11 ppm, dan ketepatan sebagai sisihan piawai relatif kira-kira 5%. Piawaian dikromat memberikan julat lineariti 0.7–1.5 ppm dengan had pengesanan dan had kuantifikasi masing-masing 0.12 dan 0.41 ppm, dan ketepatan sebagai sisihan piawai relatif di bawah 5%. Prestasi analisis ini adalah setanding tetapi tidak lebih baik daripada dua spektrofotometer komersial yang digunakan sebagai pembanding. Penggunaan spektrofotometer kamera web untuk mengukur kandungan rhodamine B dalam sampel makanan tradisional, jeli pacar cina, juga memberikan prestasi analisis yang setara, dan keupayaan pengkuantifikasian rhodamine B tidak jauh berbeza daripada spektrofotometer komersial.

 

Kata kunci: spektrofotometri nampak, spektrofotometer kamera web, prestasi analisis, analisis kuantitatif

 

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