Malaysian Journal of Analytical Sciences, Vol 27 No 3 (2023): 612 - 625

 

A VALIDATED RP-HPLC METHOD FOR THE QUANTIFICATION OF LIQUID SELF-EMULSIFYING CURCUMIN: PHARMACOKINETIC APPROACH

 

(Penentusahkan Kaedah FB-KCPT Untuk Kuantifikasi Kurkumin Pememulsi Diri Cecair: Pendekatan Farmakokinetik)

 

Xin-Yen Chiang1, Sheau-Chin Lim2, Kah-Hay Yuen1, Jia-Rou Khor1, and Siok-Yee Chan1*

 

1Departmental of Pharmaceutical Technology,

School of Pharmaceutical Sciences,

Universiti Sains Malaysia, 11800, Penang, Malaysia

2AvantSar Sdn. Bhd.,

98007, Miri, Sarawak, Malaysia

 

*Corresponding author: sychan@usm.my

 

 

Received: 26 February 2023; Accepted: 15 May 2023; Published:  23 June 2023

 

 

Abstract

The current study describes a validated, reliable, simple, and sensitive high-performance liquid chromatographic method with fluorescence detection (HPLC-FL) for the determination of curcumin at the in vitro and in vivo levels in pure and Self Emulsifying Drug Delivery System (SEDDS). The mean recovery of pure curcumin was 99.1%. The calibration curves were linear over the range from 31.3 to 1000 ng/mL with correlation coefficient of R2 ≥0.9999. The lower limit of quantification (LLOQ) was 31.3 ng/mL. The validation results confirmed the linearity, accuracy, and precision of the developed method. Furthermore, the curcumin-SEDDS excipients solubility were conducted, and later curcumin loading content (LC) was determined. Moreover, pharmacokinetic parameters, mean peak plasma concentration (Cmax), time to reach peak concentration (Tmax), and total area under the plasma concentration-time curve from time zero to the last sampling time, i.e., 1440-minute (AUC0-1440m) were successfully measured with the values of 238.81 ng/mL, 70.83 min and 11249.60 ng.min/mL, respectively. This method is suitable to detect curcumin in pure and SEDDS formulation in vitro and in vivo.

 

Keywords: curcumin, plasma, self-emulsifying drug delivery system, pharmacokinetic

 

Abstrak

Kajian semasa menerangkan kaedah kromatografi cecair (HPLC) berprestasi tinggi yang disahkan, boleh dipercayai, mudah dan sensitif dengan pengesanan pendarfluor untuk penentuan kurkumin pada tahap in vitro dan in vivo dalam Sistem Penghantaran Ubat Pengemulsi Sendiri (SEDDS) dan tulen. Purata pemulihan kurkumin tulen ialah 99.1%. Keluk penentukuran adalah linear dalam julat dari 31.3 hingga 1000 ng/mL dengan pekali korelasi R2 ≥0.9999. Had kuantifikasi yang lebih rendah (LLOQ) ialah 31.3 ng/mL. Keputusan pengesahan mengesahkan kelinearan, ketepatan dan ketepatan kaedah yang dibangunkan. Tambahan pula, keterlarutan eksipien kurkumin-SEDDS telah dijalankan, dan kandungan pemuatan kurkumin (LC) kemudiannya ditentukan. Selain itu, parameter farmakokinetik berjaya diukur dengan kaedah ini termasuk purata kepekatan plasma puncak (Cmax), masa untuk mencapai kepekatan puncak (Tmax), dan jumlah kawasan di bawah keluk masa kepekatan plasma dari masa sifar hingga masa pensampelan terakhir, iaitu, nilai 1440-minit (AUC0-1440m) masing-masing 238.81 ng/mL, 70.83 min dan 11249.60 ng.min/mL Kaedah ini sesuai untuk mengesan kurkumin dalam formula tulen dan SEDDS daripada penilaian in vitro kepada in vivo.

 

Kata kunci: kurkumin, plasma, sistem penyampaian ubat pengemulsi sendiri, farmakokinetik

 

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