Malaysian Journal of Analytical Sciences, Vol 27 No 3 (2023): 563 - 572

 

CHROMATOGRAPHIC METHODS FOR THE DETERMINATION OF DICLOFENAC IN HUMAN BIOLOGICAL SAMPLES: A MINI REVIEW

 

(Kaedah Kromatografi Untuk Penentuan Diclofenac Dalam Cecair Tubuh Manusia:

Satu Ulasan Mini)

 

Sabreenna Marsya Djuli1, and Normala Abd Latip1,2*

 

1Faculty of Pharmacy,

Universiti Teknologi MARA,

42300 Bandar Puncak Alam, Selangor, Malaysia

2Integrative Pharmacogenomics Institute (iPROMISE),

Universiti Teknologi MARA,

 42300 Bandar Puncak Alam, Selangor, Malaysia

 

*Corresponding author: drnormala6351@uitm.edu.my

 

 

Received: 2 February 2023; Accepted: 18 April 2023; Published:  23 June 2023

 

Abstract

Diclofenac is a non-steroidal anti-inflammatory drug (NSAID) used to treat pain and inflammatory disorders. Due to a general increase in the usage of pharmaceuticals and incomplete removal of such compounds from through treatment of wastewater, drugs such as diclofenac have been labelled as emerging contaminants of concern. This review covers the chromatographic methods that have been reported in recent literature for the determination of diclofenac in biological fluids of humans. This could be beneficial for researchers interested in the detection and quantification of diclofenac for epidemiological studies to determine the extent of contamination within human populations.

 

Keywords: diclofenac, chromatographic methods, human fluids

 

Abstrak

Diclofenac adalah sejenis ubatan anti-keradangan bukan steroid yang digunakan bagi merawat kesakitan dan gejala keradangan. Peningkatan penggunaan farmaseutikal dan kegagalan penyingkiran sepenuhnya sebatian farmaseutikal dari rawatan sisa buangan mengakibatkan diclofenac dilabel sebagai bahan pencemar baru muncul. Ulasan ini meliputi kaedah kromatografi yang telah dilaporkan dalam penerbitan terkini untuk penentuan kepekatan diclofenac dalam sampel cecair tubuh manusia. Ulasan ini diharap memberi faedah buat penyelidik yang melakukan pengesanan dan pengkuantitian diclofenac untuk kajian epidemiologi bagi penentuan kesan pencemaran ini kepada populasi manusia.

 

Kata kunci: diclofenac, kaedah kromatografi, sampel cecair manusia



References

1.       Alfaro, R. A. and Davis, D. D. (2021). Diclofenac. Access from https://www.ncbi.nlm.nih.gov/books/NBK557879/. [Access online 18 August 2021]

2.       Altman, R., Bosch, B., Brune, K., Patrignani, P. and Young, C. (2015). Advances in NSAID development: Evolution of diclofenac products using pharmaceutical technology. Drugs, 75: 859-877.

3.       Klopčič, I., Markovič, T., Mlinarič-Raščan, I., and Sollner Dolenc, M. (2018). Endocrine disrupting activities and immunomodulatory effects in lymphoblastoid cell lines of diclofenac, 4-hydroxydiclofenac and paracetamol. Toxicology Letters, 294: 95–104.

4.       Gröner, F., Ziková, A. and Kloas, W. (2015). Effects of the pharmaceutical’s diclofenac and metoprolol on gene expression levels of enzymes of biotransformation, excretion pathways and estrogenicity in primary hepatocytes of Nile tilapia (Oreochromis niloticus). Comparative Biochemistry Physiology Part C: Toxicology and Pharmacology, 167: 51-57.

5.       Tang, W. (2003). The metabolism of diclofenac-enzymology and toxicology perspectives. Current Drug Metabolism, 4(4): 319-329.

6.       Oaks, J. L., Gilbert, M., Virani, M. Z., Watson, R. T., Meteyer, C. U., Rideout, B. A., Shivaprasad, H. L., Ahmed, S., Chaudhry, M. J. I., Arshad, M., Mahmood, S., Ali, A. and Khan, A. A. (2004). Diclofenac residues as the cause of vulture population decline in Pakistan. Nature, 427(6975): 630-633.

7.       Hussain, I., Zargham Khan, M., Khan, A., Javed, I., and Kashif Saleemi, M. (2008). Toxicological effects of diclofenac in four avian species. Avian Pathology, 37(3): 315-321.

8.       Bonnefille, B., Gomez, E., Courant, F., Escande, A. and Fenet, H. (2018). Diclofenac in the marine environment: A review of its occurrence and effects. Marine Pollution Bullettin, 131: 496-506.

9.       Joachim, S., Beaudouin, R., Daniele, G., Geffard, A., Bado-Nilles, A., Tebby, C., Palluel, O., Dedourge-Geffard, O., Fieu, M., Bonnard, M., Palos-Ladeiro, M., Turiès, C., Vulliet, E., David, V., Baudoin, P., James, A., Andres, S. and Porcher, J. M. (2021). Effects of diclofenac on sentinel species and aquatic communities in semi-natural conditions. Ecotoxicology Environmental Safety, 211: 111812.

10.    Hunter, L. J., Wood, D. M. and Dargan, P. I. (2011). The patterns of toxicity and management of acute nonsteroidal anti-inflammatory drug (NSAID) overdose. Open Access Emergency Medicine, 3: 39-48.

11.    Sathishkumar, P., Meena, R. A. A., Palanisami, T., Ashokkumar, V., Palvannan, T. and Gu, F. L. (2020). Occurrence, interactive effects and ecological risk of diclofenac in environmental compartments and biota - a review. Science Total Environment, 698: 134057.

12.    Kołecka, K., Gajewska, M., Stepnowski, P. and Caban, M. (2019). Spatial distribution of pharmaceuticals in conventional wastewater treatment plant with Sludge Treatment Reed Beds technology. Science Total Environment, 647: 149-157.

13.    Wee, S. Y., Haron, D. E. M., Aris, A. Z., Yusoff, F. M. and Praveena, S. M. (2020). Active pharmaceutical ingredients in Malaysian drinking water: consumption, exposure, and human health risk. Environmental Geochemistry Health, 42(10): 3247-3261.

14.    Wee, S. Y., Ismail, N. A. H., Haron, D. E. M., Yusoff, F. M., Praveena, S. M. and Aris, A. Z. (2022). Pharmaceuticals, hormones, plasticizers, and pesticides in drinking water. Journal Hazardous Materials, 424: 127327.

15.    Praveena, S. M., Mohd Rashid, M. Z., Mohd Nasir, F. A., Sze Yee, W. and Aris, A. Z. (2019). Occurrence and potential human health risk of pharmaceutical residues in drinking water from Putrajaya (Malaysia). Ecotoxicology Environmental Safety, 180: 549-556.

16.    Mohd Nasir, F. A., Praveena, S. M. and Aris, A. Z. (2019). Public awareness level and occurrence of pharmaceutical residues in drinking water with potential health risk: A study from Kajang (Malaysia). Ecotoxicology Environmental Safety, 185: 109681.


17.    Leung, H. W., Jin, L., Wei, S., Tsui, M. M. P., Zhou, B., Jiao, L., Cheung, P. C., Chun, Y. K., Murphy, M. B., and Lam, P. K. S. (2013). Pharmaceuticals in tap water: Human health risk assessment and proposed monitoring framework in China. Environmental Health Perspective, 121(7): 839-846.

18.    Simazaki, D., Kubota, R., Suzuki, T., Akiba, M., Nishimura, T. and Kunikane, S. (2015). Occurrence of selected pharmaceuticals at drinking water purification plants in Japan and implications for human health. Water Research, 76: 187-200.

19.    Vulliet, E. and Cren-Olivé, C. (2011). Screening of pharmaceuticals and hormones at the regional scale, in surface and groundwaters intended to human consumption. Environmental Pollution, 159 (10): 2929-2934.

20.    Togola, A. and Budzinski, H. (2008). Multi-residue analysis of pharmaceutical compounds in aqueous samples. Journal of Chromatography A, 1177 (1): 150-158.

21.    Carmona, E., Andreu, V. and Picó, Y. (2014). Occurrence of acidic pharmaceuticals and personal care products in Turia River Basin: From waste to drinking water. Science Total Environment, 484(1): 53-63.

22.    Majors, R. E. (2013). Sample preparation fundamentals for chromatography. Agilent Technologies, Inc., Mississauga: pp 1-8.

23.    Zhou, W., Yang, S. and Wang, P. G. (2017). Matrix effects and application of matrix effect factor. Bioanalysis, 9(23): 1839-1844.

24.    Clark, K. D., Zhang, C. and Anderson, J. L. (2016). Sample preparation for bioanalytical and pharmaceutical analysis. Analytical Chemistry, 88(23): 11262-11270.

25.    Al-Bukhaiti, W. Q., Noman, A., Qasim, A. S. and Al-Farga, A. (2017). Gas chromatography: Principles, advantages and applications in food analysis. International Journal Science Innovations Discoveries, 186: 2319-1473.

26.    Szpot, P., Wachełko, O. and Zawadzki, M. (2021). Application of ultra-sensitive GC-QqQ-MS/MS (MRM) method for the determination of diclofenac in whole blood samples without derivatization. Journal of Chromatography B: Analytical Technologies Biomedicine Life Sciences, 1179: 122860.

27.    Jalbani, N. S., Solangi, A. R., Khuhawar, M. Y., Memon, S., Junejo, R., and Memon, A. A. (2020). Gas chromatographic and spectrophotometric determination of diclofenac sodium, ibuprofen, and mefenamic acid in urine and blood samples. Turkish Journal Pharmaceutical Sciences, 17: 465-473.

28.    Krokos, A., Tsakelidou, E., Michopoulou, E., Raikos, N., Theodoridis, G. and Gika, H. (2018). NSAIDs determination in human serum by GC-MS. Separation, 5(3): 37.

29.    Shah, I., Barker, J., Naughton, D. P., Barton, S. J. and Ashraf, S. S. (2016). Determination of diclofenac concentrations in human plasma using a sensitive gas chromatography mass spectrometry method. Chemistry Central Journal, 10: 52.

30.    Barfi, B., Asghari, A., Rajabi, M., Goochani Moghadam, A., Mirkhani, N. and Ahmadi, F. (2015). Comparison of ultrasound-enhanced air-assisted liquid-liquid microextraction and low-density solvent-based dispersive liquid-liquid microextraction methods for determination of nonsteroidal anti-inflammatory drugs in human urine samples. Journal Pharmaceutical Biomedicine Analysis, 111: 297-305.

31.    Harvey, D. (2019). Gas chromatography. Access from https://chem.libretexts.org/@go/page/70719. [Access online 15 March 2023]

32.    Smolková-Keulemansová, E. and Feltl, L. (1991). Comprehensive analytical chemistry. Elsevier, New York: pp. 223-462.

33.    Hage, D. S. (2018). Principles and applications of clinical mass spectrometry. Elsevier, New York: pp. 1-32.

34.    Jafari, Z. and Hadjmohammadi, M. R. (2020). A banana peel/silicon glue coated stir bar for extraction of aspirin, diclofenac, ibuprofen and mefenamic acid followed by high performance liquid chromatography-UV detection. Analytical Methods, 12: 4429-4437.

35.    Ali, S. N., Akram, S., Qayoom, A., Naz, N. and Ayub, A. (2020). Liquid chromatographic method for simultaneous determination of alprazolam with NSAIDs in bulk drug, pharmaceutical formulation and human serum. Pakistan Journal Pharmaceutical Sciences, 33: 121-127.

36.    Mirzajani, R., Kardani, F. and Ramezani, Z. (2019). Preparation and characterization of magnetic metal–organic framework nanocomposite as solid-phase microextraction fibers coupled with high-performance liquid chromatography for determination of non-steroidal anti-inflammatory drugs in biological fluids and tablet formulation samples. Microchemical Journal, 144: 270-284.

37.    Ferrone, V., Carlucci, M., Ettorre, V., Cotellese, R., Palumbo, P., Fontana, A., Siani, G., and Carlucci, G. (2018). Dispersive magnetic solid phase extraction exploiting magnetic graphene nanocomposite coupled with UHPLC-PDA for simultaneous determination of NSAIDs in human plasma and urine. Journal Pharmacutical Biomedicine Analysis, 161: 280-288.

38.    Klencsár, B., Balcaen, L., Cuyckens, F., Lynen, F. and Vanhaecke, F. (2017). Development and validation of a novel quantification approach for gradient elution reversed phase high-performance liquid chromatography coupled to tandem ICP-mass spectrometry (RP-HPLC-ICP-MS/MS) and its application to diclofenac and its related compounds. Analytica Chimica Acta, 974: 43-53.

39.    Nemoto, T., Lee, X. P., Kumazawa, T., Hasegawa, C., Fujishiro, M., Marumo, A., Shouji, Y., Inagaki, K., and Sato, K. (2014). High-throughput determination of nonsteroidal anti-inflammatory drugs in human plasma by HILIC-MS/MS. Journal Pharmaceutical Biomedicine Analysis, 88: 71-80.

40.    Dahivelkar, P. P., Bhoir, S. I., Bari, S. B., Surana, S. J. and Bhagwat, A. M. (2012). Simultaneous determination of diclofenac potassium and drotaverine hydrochloride in human plasma using reversed-phase high-performance liquid chromatography. Journal Chromatographic Science, 50: 694-701.

41.    Alpert, A. J. (1990). Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds. Journal Chromatography A., 499: 177-196.

42.    Dong, M. and Boyes, B. E. (2018). Modern trends and best practices in mobile-phase selection in reversed-phase chromatography. LC-GC Europe, 31: 572-583.

43.    Lim, C. K. and Peters, T. J. (1984). Ammonium acetate: A general purpose buffer for clinical applications of high-performance liquid chromatography. Journal of Chromatography A, 316: 397-406.

44.    Núñez, O. and Lucci, P. (2014). Applications and uses of formic acid in liquid chromatography-mass spectrometry analysis. Advances in Chemical Research, 20: 71-86.

45.    National Center for Biotechnology Information (2023). PubChem Compound Summary for CID 3033, Diclofenac. Access from https://pubchem.ncbi.nlm.nih.gov/compound/Diclofenac. [Access online 15 March 2023]

46.    Wang, Q., Wang, G., Xie, S., Zhao, X. and Zhang, Y. (2019). Comparison of high-performance liquid chromatography and ultraviolet visible spectrophotometry to determine the best method to assess Levofloxacin released from mesoporous silica microspheres/nano hydroxyapatite composite scaffolds. Experimental and Therapeutic Medicine, 17(4): 2694-2702.