Malaysian Journal of Analytical Sciences Vol 25 No 4 (2021): 569 - 583

 

 

 

 

EXTRACTION OF POMEGRANATE PEEL AND GREEN TEA LEAVES AND THEIR EFFECTS ON THE MICROBIAL, PHYSICOCHEMICAL, MICROSTRUCTURAL AND SENSORIAL PROPERTIES OF CHILLED-STORED CHICKEN MEAT

 

(Pengekstrakan Kulit Buah Delima dan Daun Teh Hijau dan Kesannya Terhadap Sifat-Sifat Mikroorganisma, Fizikokimia, Mikrostruktur dan Deria Rasa Daging Ayam yang Disimpan pada Suhu Sejuk)

 

Safiullah Jauhar1,3, Mohammad Rashedi Ismail-Fitry1*, Gun Hean Chong1, Mahmud Ab Rashid Nor-Khaizura 2, Wan Zunairah Wan Ibadullah 2

 

1Department of Food Technology, Faculty of Food Science and Technology

2Department of Food Science, Faculty of Food Science and Technology

Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

3Department of Food Technology, Faculty of Veterinary Science,

Afghanistan National Agriculture Science and Technology University, Dand District, Kandahar Province, Afghanistan

 

*Corresponding author:  ismailfitry@upm.edu.my

 

 

Received: 19 June 2021; Accepted: 27 July 2021; Published:  29 August 2021

 

 

Abstract

Pomegranate (Punica granatum L.) peel extracts (PPE) and green tea (Camellia sinensis) leaves extracts (GTE) have the potential to be the natural preservatives to prolong the chicken meat quality stored in chilling temperature. The first part of this work aimed to determine the effects of solid-liquid extraction (SLE) and ultrasound-assisted extraction (UAE) in extracting the pomegranate peel and green tea leaves. The second part was to determine the microbial, physicochemical, microstructural and sensorial properties of chicken meat applied with the PPE, GTE or PPE+GTE and stored in chill temperature for seven days. UAE method resulted in higher antioxidant activity in PPE and GTE at 74.3% and 70.4%, respectively compared to SLE method at 48.2% and 41.5%, respectively. The GTE inhibited the microbial growth with 5.47 and 5.96 log CFU/g of the chicken meat at the third and seventh day of chilled-storage, respectively. The water holding capacity, pH, lipid peroxidation and texture were not affected by the extracts. GTE affected the chicken meat by increasing the yellowness (b*), changing the microstructure, and reducing the sensory acceptability. Overall, GTE can to be used as a natural preservative for chilled chicken meat, however, further additional treatments are needed to overcome the negative effect on the chicken meat characteristics.

 

Keywords: chicken meat, green tea leaves, natural antioxidants, pomegranate peel, solid-liquid extraction, ultrasound-assisted extraction

 

 

 

Abstrak

Ekstrak kulit buah delima (Punica granatum L.) (PPE) dan ekstrak daun teh hijau (Camellia sinensis) (GTE) berpotensi menjadi pengawet semula jadi untuk mengekalkan kualiti daging ayam yang disimpan dalam suhu sejuk. Bahagian pertama kerja ini bertujuan untuk mengenalpasti kesan pengekstrakan pepejal-cecair (SLE) dan pengekstrakan secara-ultrabunyi (UAE) dalam mengekstrak kulit buah delima dan daun teh hijau. Bahagian kedua adalah untuk menentukan sifat mikroorgnisma, fizikokimia, mikrostruktur dan deria rasa daging ayam yang diaplikasikan dengan PPE, GTE atau PPE+GTE dan disimpan dalam suhu sejuk selama tujuh hari. Kaedah UAE menghasilkan aktiviti antioksida yang lebih tinggi pada PPE dan GTE masing-masing pada 74.3% dan 70.4% berbanding kaedah SLE masing-masing pada 48.2% dan 41.5%. GTE menghalang pertumbuhan mikroorganisma masing-masing dengan 5.47 dan 5.96 log CFU/g daging ayam pada hari ketiga dan ketujuh penyimpanan sejuk. Kebolehan memegang air, pH, peroksidaan lipid dan tekstur tidak dipengaruhi oleh ekstrak. GTE mempengaruhi daging ayam dengan meningkatkan warna kekuningan (b*), mengubah struktur mikro, dan mengurangkan penerimaan analisa deria rasa. Secara keseluruhan, GTE dapat digunakan sebagai pengawet semula jadi untuk daging ayam sejuk, namun, pengolahan tambahan lebih lanjut diperlukan untuk mengatasi kesan negatif kepada ciri daging ayam.

 

Kata kunci:   daging ayam, daun teh hijau, aktioksidan semulajadi, kulit buah delima, pengekstrakan pepejal-cecair, pengekstrakan  secara-ultrabunyi

 

References

1.      Casaburi, A., Di Martino, V., Ercolini, D., Parente, E. and Villani, F. (2015). Antimicrobial activity of Myrtus communis L. water-ethanol extract against meat spoilage strains of Brochothrix thermosphacta and Pseudomonas fragi in vitro and in meat. Annals of Microbiology, 65: 841-850.

2.      Giatrakou, V. and Savvaidis, I. N. (2012). Bioactive packaging technologies with chitosan as a natural preservative agent for extended shelf-life food products, modified atmosphere and active packaging technologies. Taylor & Francis, Boca Raton: pp. 685-730.

3.      Ameer, K., Shahbaz, H. M. and Kwon, J. H. (2017). Green extraction methods for polyphenols from plant matrices and their byproducts: A review. Comprehensive Reviews in Food Science and Food Safety, 16: 295-315.

4.      Pinelo, M., Ruiz-Rodríguez, A., Sineiro, J., Señoráns, F. J., Reglero, G. and Núñez, M. J. (2007). Supercritical fluid and solid-liquid extraction of phenolic antioxidants from grape pomace: A comparative study. European Food Research and Technology, 226: 199-205.

5.      Hasnaoui, N., Wathelet, B. and Jiménez-Araujo, A. (2014). Valorization of pomegranate peel from 12 cultivars: Dietary fibre composition, antioxidant capacity and functional properties. Food Chemistry, 160: 196-203.

6.      Al-Rawahi, A. (2014). Phenolic constituents of pomegranate peels (Punica granatum L.) cultivated in Oman. European Journal of Medicinal Plants, 4: 315-331.

7.      Sinija, V. R. and Mishra, H. N. (2009). Green tea: Health benefits. Journal of Nutritional and Environmental Medicine, 17: 232-242.

8.      Mitsumoto, M., O’Grady, M. N., Kerry, J. P. and Joe Buckley, D. (2005). Addition of tea catechins and vitamin C on sensory evaluation, colour and lipid stability during chilled storage in cooked or raw beef and chicken patties. Meat Science, 69: 773-779.

9.      Bozkurt, H. (2006). Utilization of natural antioxidants: Green tea extract and Thymbra spicata oil in Turkish dry-fermented sausage. Meat Science, 73: 442-450.

10.   Cong-Cong, X., Bing, W., Yi-Qiong, P., Jian-Sheng, T. and Tong, Z. (2017). Advances in extraction and analysis of phenolic compounds from plant materials. Chinese Journal of Natural Medicines, 15: 721-731.

11.   Ismail, T., Sestili, P. and Akhtar, S. (2012). Pomegranate peel and fruit extracts: A review of potential anti-inflammatory and anti-infective effects. Journal of Ethnopharmacology, 143: 397-405.

12.   Pinelo, M., Del-Fabbro, P., Manzocco, L., Nuñez, M. J. and Nicoli, M. C. (2005). Optimization of continuous phenol extraction from Vitis vinifera byproducts. Food Chemistry, 92: 109-117.

13.   Díaz-de-Cerio E., Tylewicz U., Verardo V., Fernández-Gutiérrez A. and Segura-Carretero A., (2017). Design of sonotrode ultrasound-assisted extraction of phenolic compounds from Psidium Guajava L. leaves. Food Analytical Methods, 10(8): 2781-2791.

14.   Chemat, F. and Zill-E-Huma and Khan, M. K. (2011). Applications of ultrasound in food technology: Processing, preservation and extraction. Ultrasonics Sonochemistry, 18: 813-835.

15.   Marchi, L. B., Monteiro, A. R. G., Mikcha, J. M. G., Santos, A. R., Chinellato, M. M., Marques, D. R., Dacome, A. S. and Costa, S. C. (2015). Evaluation of antioxidant and antimicrobial capacity of pomegranate peel extract (Punica granatum. L.) under different drying temperatures. Chemical Engineering Transactions, 44: 121-126.

16.   Tabaraki, R., Heidarizadi, E. and Benvidi, A. (2012). Optimization of ultrasonic-assisted extraction of pomegranate (Punica granatum L.) peel antioxidants by response surface methodology. Separation and Purification Technology, 98: 16-23.

17.   Das, P. R. and Eun, J. B. (2018). A comparative study of ultra-sonication and agitation extraction techniques on bioactive metabolites of green tea extract. Food Chemistry, 253: 22-29.

18.   Drużyńska, B., Stępniewska, A. and Wołosiak, R. (2007). The influence of time and type of solvent on efficiency of the extraction of polyphenols from green tea and antioxidant properties obtained extracts. ACTA Scientiarum polonorum Technologia Alimentaria, 6: 29-40.

19.   Wang, Z., Pan, Z., Ma, H. and Atungulu, G. G. (2011). Extract of phenolics from pomegranate peels. Open Food Science Journal, 5: 17-25.

20.   Kanatt, S. R., Chander, R. and Sharma, A. (2010). Antioxidant and antimicrobial activity of pomegranate peel extract improves the shelf life of chicken products. International Journal of Food Science Technology, 45: 216-222.

21.   Ali, U. and Kumar, P. (2015). Effect of Soxhlet and ultrasound assisted extraction on antioxidant activity of pomegranate peel extract. International Journal of Food and Nutritional Science, 3: 265-270.

22.   Al-Rawahi, A. S., Rahman, M. S., Guizani, N. and Essa, M. M. (2013). Chemical composition, water sorption isotherm, and phenolic contents in fresh and dried pomegranate peels. Drying Technology, 31: 257-263.

23.   Ghosh, M., Sinha, B. N., Seijas, J. A., Vázquez-Tato, M. P. and Feás, X. (2014). Flavonoids and phenolic compounds from Litsea polyantha juss. bark. The 18th International Electronic Conference on Synthetic Organic Chemistry, 2014: pp. 1-5.

24.   Vaithiyanathan, S., Naveena, B. M., Muthukumar, M., Girish, P. S. and Kondaiah, N. (2011). Effect of dipping in pomegranate (Punica granatum) fruit juice phenolic solution on the shelf life of chicken meat under refrigerated storage (4°C). Meat Science, 88: 409-414.

25.   Zheng, H., Han, M., Yang, H., Xu, X. and Zhou, G. (2018). The effect of pressure-assisted heating on the water holding capacity of chicken batters. Innovative Food Science and Emerging Technologies, 45: 280-286.

26.   Jauhar, S., Ismail-Fitry, M. R., Chong, G. H., Nor-Khaizura, M. A. R. and Ibadullah, W. Z. W. (2020). Application of supercritical carbon dioxide (SC-CO2) on the microbial and physicochemical quality of fresh chicken meat stored at chilling temperature. International Food Research Journal, 27(1): 103-110.

27.   Zheng, H., Xiong, G., Han, M., Deng, S., Xu, X. and Zhou, G. (2015). High pressure/thermal combinations on texture and water holding capacity of chicken batters. Innovative Food Science and Emerging Technologies, 30: 8-14.

28.   Komoltri, P. and Pakdeechanuan, P. (2012). Effects of marinating ingredients on physicochemical, microstructural and sensory properties of golek chicken. International Food Research Journal, 19: 1449-1455.

29.   Saengphol, E. and Pirak, T. (2018). Hoary basil seed mucilage as fat replacer and its effect on quality characteristics of chicken meat model. Agriculture and Natural Resources, 52(4): 382-387.

30.   Jauhar, S., Ismail-Fitry, M. R., Chong, G. H., Nor-Khaizura, M. A. R. and Ibadullah, W. Z. W. (2018). Polyphenol compounds from pomegranate (Punica Granatum) extracted via various methods and its application on meat and meat products: A review. Journal of Advanced Research in Applied Sciences and Engineering Technology12: 1-12.

31.   Pan, Z., Qu, W., Ma, H., Atungulu, G. G. and McHugh, T. H. (2012). Continuous and pulsed ultrasound-assisted extractions of antioxidants from pomegranate peel. Ultrasonics Sonochemistry, 19: 365-372.

32.   Perva-Uzunalić, A., Škerget, M., Knez, Ž., Weinreich, B., Otto, F. and Grüner, S. (2006). Extraction of active ingredients from green tea (Camellia sinensis): Extraction efficiency of major catechins and caffeine. Food Chemistry, 96(4): 597-605.

33.   Gadkari, P. V., Kadimi, U. S. and Balaraman, M. (2014). Catechin concentrates of garden tea leaves (Camellia sinensis L.): Extraction/isolation and evaluation of chemical composition. Journal of the Science of Food and Agriculture, 94: 2921-2928.

34.   Ahn, J. and Grün, I. U. and Mustapha, A. (2007). Effects of plant extracts on microbial growth, colour change, and lipid oxidation in cooked beef. Food Microbiology, 24: 7-14.

35.   Bañón, S., Díaz, P., Rodríguez, M., Garrido, M. D. and Price, A. (2007). Ascorbate, green tea and grape seed extracts increase the shelf life of low sulphite beef patties. Meat Science, 77: 626-633.

36.   Malviya, S., Arvind, Jha, A. and Hettiarachchy, N. (2014). Antioxidant and antibacterial potential of pomegranate peel extracts. Journal of Food Science and Technology, 51: 4132-4137.

37.   Chavasit, V., Photi, J., Purttiponthanee, S. and Saekoo, P. (2018). Use of bacterial growth curve for assessing risk of microbiological pathogens in food products, microbial contamination and food degradation. Academic Press, Massachusetts: pp. 341-365.

38.   El-Nashi, H. B., Abdel Fattah, A. F. A. K., Abdel Rahman, N. R. and Abd El-Razik, M. M. (2015). Quality characteristics of beef sausage containing pomegranate peels during refrigerated storage. Annals of Agricultural Sciences, 60: 403-412.

39.   Morsy, M. K., Mekawi, E. and Elsabagh, R. (2018). Impact of pomegranate peel nanoparticles on quality attributes of meatballs during refrigerated storage. LWT - Food Science and Technology, 89: 489-495.

40.   Qin, Y. Y., Zhang, Z. H., Li, L., Xiong, W., Shi, J. Y., Zhao, T. R. and Fan, J. (2013). Antioxidant effect of pomegranate rind powder extract, pomegranate juice, and pomegranate seed powder extract as antioxidants in raw ground pork meat. Food Science and Biotechnology, 22: 1063-1069.

41.   Jay, J. M. (1998). Modern food microbiology (Fifth Edition). Aspen Publishers, Inc., Maryland: pp. 38-41.

42.   Turgut, S. S., Soyer, A. and Işıkçı, F. (2016). Effect of pomegranate peel extract on lipid and protein oxidation in beef meatballs during refrigerated storage. Meat Science, 116: 126-132.

43.   Rababah, T. M., Ereifej, K. I., Al-Mahasneh, M. A. and Al-Rababah, M. A. (2006). Effect of plant extracts on physicochemical properties of chicken breast meat cooked using conventional electric oven or microwave. Poultry Science, 85(1): 148-154.