Malaysian Journal of Analytical Sciences Vol 25 No 3 (2021): 508 - 520

 

 

 

 

THE EFFECTS OF STORAGE TEMPERATURE AND TIME ON THE LEVELS OF PHTHALATES IN COMMERCIAL PET-BOTTLED WATER

 

(Kesan Suhu dan Masa Penyimpanan Terhadap Kandungan Ftalat dalam Air Berbotol PET Komersial)

 

Nur Amira Syazwan Razali¹, Ungku Fatimah Ungku Zainal Abidin^2,3, Nur Hanani Zainal Abedin^1,3, Syaliza Omar⁴, Jinap Selamat^1,5, Maimunah Sanny^1,5*

 

¹Department of Food Science, Faculty of Food Science and Technology

²Department of Food Service Management, Faculty of Food Science and Technology

³Halal Products Research Institute,

Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

⁴Faculty of Pharmacy,

Universiti Sultan Zainal Abidin, Besut Campus, 222000, Besut, Terengganu, Malaysia

⁵Laboratory of Food Safety and Food Integrity, Institute of Tropical Agricultural and Food Security,

Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

 

*Corresponding author:  s_maimunah@upm.edu.my

 

 

Received:  5 April 2021; Accepted: 22 May 2021; Published:  27 June 2021

 

 

Abstract

This study was conducted to determine the effects of storage temperature and time on the levels of phthalates in commercial PET-bottled water. Freshly-produced water samples consisted of drinking, mineral, and sparkling in PET bottles were collected from a manufacturing site and subjected to different storage temperatures (refrigeration temperature of 4 °C, room temperature of 25 °C, and 40 °C) and times (0 month as control, 1.5 months, 3 months, and 6 months). Six different phthalates were analyzed using LC-MS/MS with deuterated bis(2-ethylhexyl)phthalate (DEHP) as internal standard. DEHP in the PET-bottled water was detected in the range from 2.32 to 27.6 ng/mL for 3- and 6-month storage samples; higher than di-n-octyl phthalate (DnOP) detected in the range from 1.57 to 12.6 ng/mL. Higher levels of DEHP and DnOP in PET-bottled mineral water were detected at room temperature of 25 °C when compared to refrigeration temperature of 4 °C, and 40 °C at 6-month storage. Higher level of DEHP and DnOP in drinking water was found at 6 months compared to 3 months in refrigeration temperature of 4 °C.  The pronounced effects of storage temperatures on the levels of phthalates was observed only after 6 months of storage in which DEHP levels exceeded the maximum established limit of 6 ng/mL.

 

Keywords: PET-bottled water, phthalates, storage temperature, storage time, liquid chromatography-mass spectrometry/mass spectrometry.

 

Abstrak

Kajian ini dilakukan untuk mengetahui kesan suhu dan masa penyimpanan terhadap kandungan phthalates dalam air berbotol PET komersial.  Sampel air yang baharu dihasilkan di pusat pengilangan terdiri daripada air minuman, air mineral, dan air soda yang dibotolkan dengan menggunakan botol PET telah dikumpulkan dan disimpan di dalam suhu (suhu penyejukan 4 °C, suhu bilik 25 °C, dan 40 °C) dan masa (0 bulan sebagai kawalan, 1.5 bulan, 3 bulan, dan 6 bulan) yang berbeza. Enam jenis ftalat dianalisa dengan menggunakan LC-MS/MS dan bis(2-etilheksilt)ftalat (DEHP) deuterasi sebagai piawai dalaman. DEHP dalam air botol PET dikesan dalam julat 2.32 hingga 27.6 ng/mL untuk sampel penyimpanan 3- dan 6 bulan; lebih tinggi daripada di-n-oktil ftalat (DnOP) yang dikesan dalam julat 1.57 hingga 12.6 ng/mL. Tahap DEHP dan DnOP yang lebih tinggi dalam air mineral botol PET dikesan pada suhu bilik 25 °C jika dibandingkan dengan suhu penyejukan 4 °C, dan 40 °C pada penyimpanan 6 bulan. Tahap DEHP dan DnOP yang lebih tinggi dalam air minuman didapati pada 6 bulan berbanding 3 bulan pada suhu penyejukan 4 °C. Kesan suhu penyimpanan yang ketara kepada kandungan ftalat diperhatikan hanya selepas penyimpanan selama 6 bulan di mana tahap DEHP melebihi had maksimum yang ditetapkan iaitu 6 ng/mL.

 

Kata kunci:  air berbotol PET, ftalat, masa penyimpanan, suhu penyimpanan, kromatografi cecair-spektrometri jisim/spektrometri jisim

 

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