Malaysian Journal of Analytical Sciences Vol 18 No 3 (2014): 683 - 689

 

 

 

FTIR ANALYSIS OF PHENOLIC COMPOUND AS PANCREATIC LIPASE INHIBITOR FROM INOCULATED AQUILARIA MALACCENSIS

 

(Analisis Sebatian Fenolik daripada Aquilaria malaccensis yang di Inokulasi sebagai Perencat kepada Enzim Lipase Pankreas Menggunakan FTIR)

 

Nur Fahana Jamahseri*, Miradatul Najwa Mohd Rodhi, Nur Hidayah Zulkarnain, Nursyuhada Che Husain,

 Ahmad Fakhri Syahmi Masruddin

 

Faculty of Chemical Engineering,

Universiti Teknologi MARA, 40450 Shah Alam, Selangor Darul Ehsan, Malaysia

 

*Corresponding author: farah_hana90@ymail.com

 

 

Abstract

This research aimed to discover the potential of inoculated Aquilaria malaccensis extract as a new and safe lipase inhibitor. The phenolic compounds in this plant are expected to promote inhibitory activity towards pancreatic lipase enzyme. Inoculated Aquilaria malaccensis was selected for this research, wherein the parts of this species (bark and leaves) were extracted via hydro distillation process. The extracts of this plant which are hydrosol, oil, and leaves were analyzed for phytochemical compound via Fourier Transform Infrared Spectroscopy (FTIR). FTIR analysis of the extracts of inoculated Aquilaria malccensis revealed the presence of hydroxyl functional group in both leaves and hydrosol extracts but absence in oil. This validate the presence of phenolic compound in hydrosol and leaves extract. Therefore, the leaves and hydrosol extracts have potential as an anti-obesity agent by inhibiting pancreatic lipase.

 

Keywords: Aquilaria malaccensis, phenolics, essential oil, hydrosol

 

Abstrak

Kajian ini dijalankan bertujuan mengenal pasti potensi ekstrak Aquilaria malaccensis yang diinokulasi sebagai inhibitor enzim lipase pankreas yang baru dan selamat. Sebatian fenolik dalam tumbuhan ini dipercayai akan dapat merencatkan aktiviti enzim lipase pankreas. Bahagian tumbuhan iaitu kayu dan daun telah diekstrak dengan menggunakan kaedah penyulingan hidro. Penyulingan hidro pada bahagian kayu tumbuhan menghasilkan minyak dan hidrosol.Ekstrak daripada kayu dan daun kemudian dianalisis untuk sebatian fitokimia menggunakan Spektrofotometer Inframerah Transformasi Fourier (FTIR). Melalui analisis FTIR, kumpulan berfungsi hidrosil didapati pada sampel ekstrak daun dan hidrosol namun tidak pada ekstrak minyak. Kajian ini mengesahkan kehadiran sebatian fenolik pada ekstrak daun dan hidrosol. Oleh itu, ekstrak daun dan hidrosol mempunyai potensi sebagai agen anti -obesiti dengan merencatkan aktiviti enzim lipase pankreas.

 

Kata kunci: Aquilaria malaccensis, fenolik, minyak, hidrosol

 

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