Malaysian Journal of Analytical Sciences Vol 18 No 2 (2014): 351 – 359

 

 

 

PHYTOCHEMICAL SCREENING, TOTAL PHENOL ESTIMATION, ANTIOXIDANT ACTIVITY OF BLAINVILLEA ACMELLA LEAF AND STEM SUCCESSIVE EXTRACTS

 

(Ujian Penyaringan Fitokimia, Penganggaran Fenol dan Aktiviti Antioksidan Blainvillea Acmella dan Ekstrak Berturutan Daun dan Stem)

 

Piush Sharma¹*, Ganesh N. Sharma², B. Shrivastava², Hemant R. Jadhav³

 

¹Maharishi Arvind College of Pharmacy,

Ambabari, Jaipur (Raj). India-302023

²School of Pharmaceutical Sciences,

Jaipur National University, Jaipur (Raj) India-302025

³Department of Pharmacy,

Birla Institute of Technology & Science, Pilani, Rajasthan, India-333031

 

*Corresponding author: joshipiush@gmail.com

 

 

Abstract

The aim of present work was to investigate antioxidant potential of different extracts of Blainvillea acmella leaf and stem. The successive extraction of individual plant part was carried out using solvents of different polarity viz. n-hexane, ethyl acetate, methanol and water. Preliminary phytochemical screening of all the extracts was done. The present total phenolic contents were estimated by Folin-Ciocalteu reagent method and expressed as µg/mg of gallic acid equivalent. The antioxidant potential and reducing power of all the prepared extracts were measured against DPPH, as compared to standard ascorbic acid, and BHA respectively. The result data indicate that the phenolic contents were higher in methanolic extracts of leaf (73.67±0.38mg/g) followed by ethyl acetate (29.08±0.38mg/g), aqueous (21.50±0.28 mg/g), and n-Hexane (9.29±0.38 mg/g); gallic acid equivalent. The similar pattern in stem part was also observed i.e. methanolic extracts (41.90±0.45mg/g), ethyl acetate (21.92±0.28 mg/g), aqueous (15.13±0.18mg/g), and n-Hexane (3.69±0.28 mg/g). The antioxidant capacity of methanolic extract of both the part i.e leaf and stem was found to be maximum, as IC50 values were 226.49±0.16, 402.05±1.10 respectively. The reducing power was also highest in methanol extract of both parts. The result data conclude that the higher antioxidant as well as reducing power may be due to present phenolic contents.

 

Keywords: Blainvillea acmella, Antioxidant, Reducing Power, DPPH, IC50

 

Abstrak

Kajian ini bertujuan meneliti potensi bahan antioksidan dari ekstrak daun dan batang Blainvillea acmella yang berbeza. Pengekstrakan berturutan pada bahagian tertentu tumbuhan telah dijalankan dengan menggunakan pelarut berkutub berbeza seperti n-heksana, etil asetat, metanol dan air. Saringan awal fitokimia terhadap semua sampel ekstrak telah dilakukan. Jumlah kandungan fenolik telah dianggar dengan  mengunapakai kaedah reagen Folin-Ciocalteu dan dinyatakan sebagai kesetaraan μg/ mg asid Gallic. Potensi antioksidan  dan kuasa penurun bahan sampel terekstrak diukur melawan DPPH, sebagai perbandingan masing – masing terhadap asid askorbik piawai dan BHA. Data kajian menunjukkan bahawa kandungan fenolik adalah lebih tinggi di dalam daun mengunakan pengekstrak metanol (73.67 ± 0.38mg /g) diikuti etil asetat (29.08 ± 0.38mg /g), air (21.50 ± 0.28 mg /g) dan n-heksana (9.29 ± 0.38 mg/g) kesetaraan asid Gallic. Corak yang sama di bahagian batang  juga diperhatikan iaitu ekstrak metanol (41.90 ± 0.45mg /g), etil asetat (21.92 ± 0.28 mg/g), air (15.13 ± 0.18mg/ g), dan n-Heksana (3.69 ± 0.28 mg/g). Kapasiti antioksidan mengunakan pengekstrak metanol daripada kedua-dua bahagian iaitu daun dan batang didapati maksimum, dengan nilai IC50 masing – masing adalah  226.49 ± 0.16 dan 402.05 ± 1.10. Kuasa pengurnagn juga paling tinggi di dalam ekstrak metanol bagi kedua-dua bahagian. Kesimpulan bahawa kehadiran kandungan fenolik menyebabkan nilai antioksidan dan kuasa pengurangan menjadi lebih tinggi.

 

Kata kunci: Blainvillea acmella, Antioksidan, Kuasa penurun, DPPH, IC50

 

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