Malaysian Journal of Analytical Sciences Vol 18 No 1 (2014): 58 – 67

 

 

 

PHOSPHORYLATION OF GELATINE AND CHITOSAN AS AN EXCIPIENT FOR ASIATICOSIDE NANOFIBERS

 

(Pemfosforilan Gelatin dan Kitosan Sebagai Eksipien Bagi Penyediaan Nanofiber Asiatikosida)

 

Susana Elya Sudrajat¹*, Khairurrijal², Puspa DN Lotulung³, Effionora Anwar¹

 

¹Faculty of Pharmacy,

Universitas Indonesia, Depok, Indonesia

²Department of Physics,

Institut Teknologi Bandung, Indonesia

³Department of Chemistry,

Lembaga Ilmu Pengetahuan Indonesia, Serpong, Indonesia

 

*Corresponding author: susanasudrajat_09@yahoo.com

 

 

Abstract

Asiaticoside has been widely used and is still studied for topical treatment of wounds, increased fibroblasts and collagen production are noted at the site of injury. Base on getting the optimal effect of wounds’ treatment, drug must be released and absorbed from the carrier/drug delivery media. In this research, nanofibers preparation is made using the model drug asiaticoside. The result indicated that gelatine-chitosan  phosphorylation can be used as an excipient for asiaticoside nanofibers. In this research 3 formulas with varying concentrations of phosphorus are used. The release of asiaticoside was examined by dissolution in vitro. It was measured by high performance liquid chromatography (HPLC) and nanofibers morphology was measured by scanning electron microscopy (SEM). Phosphorylation chitosan tested by infra red spectrophotometry (FTIR) at wave numbers 1271, 1213, 1157, 1085, 1012 and 954 cm^-1 where as the phosphorylation of gelatine at 1257, 1026 and 900 cm^-1 . The characterization result indicates that the formula C was contains sodium tripolyphosphate  0.5% is the best with the release of asiaticoside 51% for 72 hours, compared to formula A(68%) and the formula B(62%). The percentage asiaticoside from nanofibers preparation is between 90%-100%.

 

Keywords: asiaticoside, nanofibers, phosphorylation, sodium tripolyphosphate, electrospinning, dissolution

 

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