Malaysian Journal of Analytical Sciences Vol 18 No 1 (2014): 212 - 220

 

 

 

DEVELOPMENT OF SPECTROPHOTOMETRIC METHOD

FOR ALLOPURINOL IN URINE BASED ON THE DIAZOTIZATION REACTION

 

(Pembangunan Kaedah Spektrofotometrik untuk Penentuan Allopurinol dalam Air Kencing Berdasarkan Reaksi Diazotisasi)

 

Edy Agustian Yazid¹*, Ganden Supriyanto^1,2, Tjitjik Srie Tjahjandarie¹

 

¹Chemistry Depertment, Faculty of Science and Technology,

²Institute of Tropical Disease,

Airlangga University, Surabaya, Indonesia

 

*Corresponding author: estien_y@yahoo.co.id

 

 

Abstract

A simple, rapid, low cost, accurate and sensitive spectrophotometric method for the determination of allopurinol (ALP) in urine was developed. This method is based on the reaction of diazotized sulfanilamide with ALP as coupling agent in an alkaline medium. The resulting intense yellow dye has a maximum absorption at 400 nm and stable for more than 1 hour. The Beer’s law limits was obeyed over the concentration range of ALP in aqueous medium from 5.0-50 mg/mL. The molar absorptivity and Sandell’s sensitivity were obtained 2.5 × 10³ L mol^–1 cm^–1 and 0.061 μg cm^–2 /0,001 absorbance unit, respectively. The optimum reaction conditions and other analytical parameters have also been investigated. The correlation coefficient (r) was obtained 0.999. LOD and LOQ were found 2.2 μg/mL and 7.5 μg/ mL, respectively. The percent recovery and relative standard deviation (RSD) were found 99.18-100.10 % and 0.3-1.3 %, respectively. The proposed method can be applied successfully to assay ALP in urine by standard addition method and can be considered as an alternative method for determination ALP in the urine samples.

 

Keywords: spectrophotometry, allopurinol, sulfanilamide, diazotization reaction, coupling

 

References

1.       Ruiz, T.P., Lozano, C.M., Tomas, V. & Martin, J., (2003). Determination of allopurinol by micelle-stabilised room temperature phosphorescence in real samples. Journal of Pharmaceutical and Biomedical Analysis, 32: 225–231.

2.       Amal A. Ammar, A.A., Samy, A.M., Marzouk, M.A., Ahmed, M.K., (2011). Formulation, Characterization And Biopharmaceutical Evaluation Of Allopurinol Tablets, International Journal of Biopharmaceutics, 2(2): 63-71.

3.       Sreeram, V., Rao, M.V.B., Karumuri, S.R., (2013), A Validated and Stability Indicating Ultra Performance Liquid Chromatographic Method for Allopurinol in Pharmaceutical Preparation, Paripex-Indian Journal Of Research, 3: 6–10.

4.       Reinders, M.K., Nijdam, L.C.,Van-Roon, E.N., Movig, K.L.L., Jansen, T.L.Th.A., Van De Laar, M.A.F.J., Brouwers, J.R.B.J., (2007). A Simple Method for Quantification of Allopurinol and Oxipurinol in Human Serum by High-Performance Liquid Chromatography with UV-Detection, Journal of Pharmaceutical and Biomedical Analysis, 45(2): 312-317.

5.       Ali, N.W., Abdelwahab, N.S., EL-fatatry, H.M., and Osman, W.M., (2013). Spectrophotometric Methods for Simultaneous Determination of Two Hypouricemic Drugs in their Combined Dosage Form. Pharm Anal Acta, 4(6): 2-8.

6.       Purwanto, B., (2012). Supplementation Allopurinol Pre-Exercise For Athletes Safeguard Against Oxidative Stress, IPTEKOR Journal, 5 (1) : 1 - 11.

7.       Chevion, S., Moran, D.S., & Heled, Y., (2003). Serum Antioxidant Stress and Cell Injury After Severe Physicaal Exercise, Proceedings of The United State of America, 100 (9): 5119-5123.

8.       Cortina-Puig, M., & Camp, M., (2007). Electrochemical Biosensors as a Tool for Antioxidant Capacity Assessment, J Sens Actua, 129: 459-466.

9.       Small, D.M., Coombes, J., Bennett, M., Johnson, D.W., and Gobe, G.C., (2012). Oxidative Stress, Anti-Oxidant Therapies and Chronic Kidney Disease, Asian Pacific Society Of Nephrology 17(4): 311-321.

10.    Halliwell. B., Gutteridge J.M.C., (2007). Free Radical in Biology and Medicine, 4^th ed, Oxford University Press, New York.

11.    Graham, S., Day, R.O., Wong, H., Mclachlan, A.J., Bergendal, L., Miners, J.O., & Birkett, D.J., (1996). Pharmacodynamics of Oxypurinol After Administration of Allopurinol to Healthy Subjects, Br J Clin Pharmacol, 41: 299–304.

12.    George, J., Carr, E., Davies, J., Belch, J.J., Struthers, A., (2009). High-Dose Allopurinol Improves Endothelial Function by Profoundly Reducing Vascular Oxidative Stress and Not by Lowering Uric Acid, Circulation, 114: 2508–2516.

13.    Hassib, S.T., Safwat, H.M., and El-Bagry. R.I., (1986). Spectrophotometric Determination of Some Anti-Inflammatory Agents Using N-Bromosuccinimide, Analyst, 111: 45-48.

14.    Khayoon, W.S., M.Q. Al-Abaichy, M.Q., Jasim, M, and M.A. Al-Hamadany, M.A., (2008). Spectrophotometric Determination of Allopurinol in Tablet Formulation, Journal. Phys. Sci., 19(2): 23-30.

15.    Ruiz, T.P., Lozano, CM., Tomás, V., and  Galera, R., (2003). Development of a Capillary Electrophoresis Method for The Determination of Allopurinol And its Active Metabolite Oxypurinol, Journal Chromat. B, 798: 303–308.

16.    Sun, X., Cao, W., Bai, X., Yang, X., Wang, E., (2001). Determination of Allopurinol and its Active Metabolite Oxypurinol by Capillary Electrophoresis with End-Column Amperometric Detection, Analytica Chimica Acta, 121–128.

17.    Zen, J.M., Chen, P.Y., and Kumar, A.S., (2002). Flow Injection Analysis of Allopurinol by Enzymeless Approach at Glassy Carbon Electrodes, Electroanalysis, 14 (10): 645-649.

18.    Cataldi, T.R.I., Palmisano, F., Zambonin, P.G., (1989). Flow Injection with Anodic Polarographic Detection for The Determination of Allopurinol in Pharmaceutical Formulations, Analyst., 114(1989): 1449- 1452.