Malaysian Journal of Analytical Sciences Vol 18 No 1 (2014): 107 – 115

 

 

 

HYDROGEN PEROXIDE IMPEDIMETRIC DETECTION ON POLY-ORTHO-PHENYLENEDIAMINE MODIFIED PLATINUM DISK MICROELECTRODE

 

(Pengesanan Hidrogen Peroksida Secara Impedimetrik Di Atas Elektrod Mikro Cakera Platinum Yang Di Ubah Suai Dengan Poli-Orto-Fenilinadiamina)

 

Zainiharyati Mohd Zain* and Norazreen Zakaria

 

Faculty of Applied Sciences,

Universiti Teknologi MARA, 40450 Shah Alam,  Selangor, Malaysia

 

*Corresponding author: zainihar@salam.uitm.edu.my

 

 

Abstract

This work describes the development of hydrogen peroxide detection based on Poly-ortho-phenylenediamine modified Platinum disk microelectrode (50 µm in diameter).  The electrochemical performances of H₂O₂ detection were studied using Chronoamperometry, Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) techniques in Phosphate Buffer Solution (PBS) pH 7.4.  Effect of potential, electrode size, and various concentrations of H₂O₂, among others, were investigated by tracking the impedance changes at a specific perturbation frequency. To obtain the Charge transfer resistance (R_ct) values, a modified Randles Equivalent Circuit was modelled and fitted to Nyquist Plot. Then, this sensor was further applied in the detection of H₂O₂ in antiseptic mouthwash with percent recovery of 97% ± 0.14 (x10³ kΩ).

 

Keywords:   Electrochemical Impedance Spectroscopy, Platinum microelectrode,  hydrogen peroxide, poly-ortho-phenylen-diamine

 

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