The Malaysian Journal of Analytical Sciences Vol 15 No 2 (2011): 175 – 183

 

 

 

DETERMINATION OF ORGANOPHOSPHORUS PESTICIDES

 USING MOLECULARLY IMPRINTED POLYMER

 SOLID PHASE EXTRACTION

 

Mohd Marsin Sanagi^1,2*, Syairah Salleh², Wan Aini Wan Ibrahim^1,2, Ahmedy Abu Naim¹

 

¹IbnuSina Institute for Fundamental Science Studies,

Nanotechnology Research Alliance,

²Department of Chemistry, Faculty of Science,

UniversitiTeknologi Malaysia, 81310 UTM JohorBahru, Johor, Malaysia

 

*Corresponding author: marsin@kimia.fsutm.my

 

 

Abstract

Molecularly imprinted polymer solid phase extraction (MIP-SPE) method has been developed for the determination of organophosphorus pesticides (OPPs) in water samples. The MIP was prepared by thermo-polymerization method using methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as crosslinker, acetonitrile as porogenic solvent and quinalphos as the template molecule. The three OPPs (diazinon, quinalphos and chlorpyrifos) were selected as target analytes as they are widely used in agriculture sector.  Various parameters affecting the extraction efficiency of the imprinted polymers have been evaluated to optimize the selective preconcentration of OPPs from aqueous samples.  The characteristics of the MIP-SPE method were validated by high performance liquid chromatography (HPLC). The accuracy and selectivity of the MIP-SPE process developed were verified using non-imprinted polymer solid phase extraction (NIP-SPE) and a commercial C₁₈-SPE was used for comparison. The recoveries of the target analytes obtained using the MIPs as the solid phase sorbent ranged from 83% to 98% (RSDs 1.05 - 1.98%; n=3) for water sample.  The developed MIP-SPE method demonstrates that it could be applied for the determination of OPPs in water samples.

 

Keywords: Organophosphorus pesticides, Molecularly imprinted polymer, Solid phase extraction

 

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