The Malaysian Journal of Analytical Sciences Vol 15 No 1 (2011): 54 – 60

 

 

 

A  SIMPLE  AND  COST  EFFECTIVE  ISOLATION AND PURIFICATION  PROTOCOL OF  MITRAGYNINE  FROM MITRAGYNA  SPECIOSA  KORTH  (KETUM)  LEAVES

 

Goh Teik Beng^1* , Mohamad Razak Hamdan¹, Mohammad Jamshed Siddiqui², Mohd Nizam Mordi¹

and Sharif Mahsufi Mansor¹

 

¹Centre of Drug Research,

² School of Pharmaceutical Science,

Universiti Sains Malaysia, Minden 11800, Penang, Malaysia.

 

*Corresponding author: gtb.dd04@student.usm.my

 

 

Abstract

The objective of the present study was to develop a simple and cost effective method for the isolation of mitragynine from Mitragyna speciosa Korth leaves. The results of our study showed that around 0.088 % (w/w) pure mitragynine was obtained by this newly  developed method with a purity of 99.0 % (w/w) based on GC-MS analysis. Moreover, the recovery of the pure mitragynine from the chloroform extract was also more than 95.0 %. Advantages of this new method were simpler, faster and more economical. In conclusion this simple and cost effective method helps to isolate mitragynine with higher purity in comparison with the published methods.

 

Keywords: Mitragynine speciosa Korth,  mitragynine,  mitragynine extraction

 

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