The Malaysian Journal of Analytical Sciences Vol 15 No 1 (2011): 113 – 121

 

 

 

ANALISIS KROMATOGRAFI GAS x KROMATOGRAFI GAS−SPEKTROMETRI JISIM MASA-PENERBANGAN DAN AKTIVITI ANTIBAKTERIA MINYAK PATI AMOMUM XANTHOPHLEBIUM

 

(Gas Chromatography x Gas Chromatography–Time-of-Flight Mass Spectrometry Analysis and Antibacterial Activity of Essential Oil from Amomum xanthophlebium)

 

A. Masila¹, I. Aminah¹, W.A. Yaacob¹, I. Nazlina²

 

¹Pusat Pengajian Sains Kimia dan Teknologi Makanan,

²Pusat Pengajian Biosains dan Bioteknologi,

Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia,

43600 Bangi, Selangor, Malaysia.

 

*Corresponding author: wanyaa@ukm.my

 

 

Abstract

Essential oils of fresh leaves, stem, rhizomes and whole aromatic plants of Amomum xanthophlebium (Zingiberaceae) were obtained by hydrodistillation. Percentage yields of the leaf, stem and whole plant oils were 0.0032, 0.0074 and 0.0021% whereas the rhizome oil obtained was very little. Chemical components of each oil and their percentages were determined by Gas Chromatography x Gas Chromatography–Time-of-Flight Mass Spectrometry (GCxGC-TOFMS). Analysis of A. xanthophlebium oils showed that they were dominated by terpenes. Main components in the leaves were allo-aromadendrene (3.41%), (±)-globulol (2.58%) and rosifoliol (2.55%); stem, α-terpineol (4.25%), rosifoliol (2.41%) and bingpian (2.27%); rhizomes, viridiflorol (5.72%), (±)-globulol (5.23%) and α-cadinol (4.81%); whole plants, eucalyptol (4.11%), l-α-terpineol (2.88%) and rosifoliol (2.82%). The stem oil of A. xanthophlebium showed antibacterial activity against Gram-negative Escherichia coli and Gram-positive methicillin-resistant Staphylococcus aureus (MRSA) at the minimum inhibitory concentration of 80 mg/ml.

 

Keywords: Amomum xanthophlebium, essential oil, GCxGC−TOFMS

 

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